Aqueous Leaves Extract of <i>Gongronema latifolium</i> (Benth) Downregulates the Expression of IFN-γ, IL-10 and Cell Surface Markers in Rabbits

Background: The pathophysiology of the inflammatory process reveals intricate signaling which includes IL-1β, IL-6, and TNFα pathways that could serve as drug targets. Aim: This study determined effect aqueous extract Gongronema latifolium (AEGL) leaves on expression IFNγ, IL-10, CD3, CD56 in rabbits. Materials Methods: ELISA tests were performed to determine AEGL a pro-inflammatory cytokine (IFNγ), an anti-inflammatory (IL-10), CD3 cell surface markers Twenty cross-bred male rabbits with average weight range 1.0 - 1.5 kg selected. separated into four groups each treated follows: Grp1 is untreated control; Grp2 Grp3, Grp4, Grp5 200, 400, 600 mg/kg respectively for 28 days. Results: showed its greatest inhibitory Group 4 IL-10 (118.5 pg/ml), IFNγ (332 pg/ml) days 14 21 respectively. also highest inhibition (0 3; day (630.5 4. Conclusion: exhibited strong T mediated anti- inflammatory, immunomodulatory activity test within 28-day period can be confirmed by based assays. Specifically at 400 mg/kg, suggestive maximum effective dose.